DT excavator notes:
In the previous article, we exposed the factual clues that the CCP used the state apparatus to kidnap 1.4 billion Chinese people to steal US scientific and technological achievements and talents. We also explained the implementation method and process of the “BGY” plan through several case studies. It can be said that through various BGY means, they almost achieved their purpose of 3F America. Is the novel coronavirus the only weapon in the planned development of “Viral bioweapons”, a core part of the shipwreck plan against the United States? Absolutely not of course. The CCP organized a huge scientific research team, established two P4 safety level biological laboratories and many P3 level biological safety laboratories. This means that they prepared a huge strategic reserve, the purpose is not a weapon, but many more biological weapons.
Part 2: Animal labs and animals for conducting animal model tests.
Having clarified that the number of viral strains at the disposal of the P4 laboratory has a huge volume of more than 60,000 strains in more than 1,400 categories, we will first focus on the animal and animals laboratories that conduct animal model tests.
Laboratory animals are important because they are essential in the first step in the development of viral genetic weapons that must be tested in animal experiments and animal tests. First of all, we need to clarify the difference between experiments, which are conducted in a laboratory to obtain experimental data, and tests, which are conducted to detect viral weapons’ effect. Animal experiments generally include low-level experimental animals such as mice (for experimental use) and high-level experimental animals (human-like primates) also known as Various monkeys. Animal tests do not necessarily use real viruses, and similar viruses (same principle virus) can be used to test the effect, but this test must be in a natural environment, such as on natural bats, natural farmed livestock like pigs, natural farmed monkeys, and even on a real human. Since this is a trial, the danger and secrecy will be high. Precautions and preparations should be perfect. The reason that we have concluded that the swine fever outbreak in the remote mountainous areas of Guangzhou in 2016 was an testing (not experiment) of coronavirus weapons.
Without going into more detail about experimental animals, here is just a digression into the higher levels of primates in experimental animals, as only this kind of human-like animal can only be used for viral weapon experimentation and testing. Simply put, after you have verified that this virus can be transmitted to monkeys in the lab, and proven through field trials that the virus can infect monkeys by aerosol (the main mode of transmission of the virus), it can be easily concluded that the manufacturing virus is a zoonotic infectious virus now. And since the swine fever virus of 2016 has already found that group of pigs, it’s critical to find the monkeys. Of course, it’s impossible to go to a nature reserve to find the monkeys, it’s risky, and there could be another kind of AIDS Virus.
They found the monkeys after SARS back in 2003.
Let’s start with three tables.
Biological Weapons Super Team
2003 SARS Tasks Division
Chinese Academy of Sciences and major military system units involved in the fight against SARS in 2003
All three of these forms have been shown earlier and are significant to the search for monkeys, the last of which is the basis of the second, while the second table is the basis for the first. Then in the third table, it has been clearly shown that the Kunming Animal Institute and the three units of the Institute of Military Medical Sciences and the General Logistics Department of the PLA, are establishing a higher animal research center. This is the “Primate Experimental Animal Center” at the Kunming Institute of Zoology, Chinese Academy of Sciences, where the monkeys are located.
The article makes it clear that the “Primate Centre” has been in existence since 2001 and that it was established in 2003 before SARS. And this task was not carried out by the Kunming Animal Institute alone, the specific cooperation can be found in that speech by Lu Yongxiang: “At the same time The Kunming Institute of Zoology and research institutes in Shanghai, together with the Academy of Military Medical Sciences, and with the support of the PLA General Logistics Department, organized the establishment of the Higher Animal Research Center, using primates to conduct virology experiments. The Animal Institute is self-funded to conduct research in Guangdong to find the source of pathogens.” In other words, the division of labor among the specific units in the above table: with the support of the PLA’s General Logistics Department (Safety and Security), the Kunming Animal Institute and Shanghai, together with the Academy of Military Medical Sciences, organized the establishment of the Centre for Advanced Animal Research. Our excavations clearly show that the real managers and users of the High Animal Research Center, which is based on the Kunming Institute of Zoology. Academy of Military Medical Sciences and Shanghai, and the subsequent inconsistency in the number of experimental monkeys proves it.
This is an introduction to the Kunming Animal Clinic on the official website.
Primate experimental animal center, Kunming Institute of Zoology, Chinese Academy of Sciences.
Kunming Institute of Zoology, Chinese Academy of Sciences, was the first in China to carry out primate domestication, breeding and research, established in 1959. It’s the first artificial primate breeding center in China with a population of rhesus macaques, crab-eating monkeys, short-tailed monkeys, bear monkeys, capuchin monkeys, golden snub-nosed monkeys and tree shrews. Now there are a variety of primate experimental animal populations, including macaques, crab-eating monkeys, short-tailed monkeys, bear monkeys, capuchin monkeys, golden snub-nosed monkeys and tree shrews, etc., which can be used to breed a variety of primate species every year, produce hundreds of high quality experimental primates with “ID cards” and a clear genetic background. The computer manages the archive. In the picture, those red brick houses that stand out under the white lines are the “Monkey Villa”. The beauty of the environment immediately reminds us of Sun Wukong’s “Mountain of Flowers and Fruits”. Except for the staff, no one is allowed to come and go, which is why it is said to be crowded but rarely visited.
After decades of construction, the Kunming Institute of Zoology of the Chinese Academy of Sciences has become one of the best primate biology research units in China. There is a primate breeding animal room with a floor area of 5500 square meters and 3 animal laboratory buildings with a floor area of 6500 square meters. High-grade primate laboratories with a cleanliness level of 10,000, these laboratories can accommodate 500 primate experiments, while meeting the screening and pharmacokinetic experiments of 12 new drugs. We have built an animal experimental technology system to support basic research on the mechanisms of major human diseases, research and construction of disease animal models, and the development of new drugs. It is a public service platform for pharmacokinetic evaluation and preclinical safety evaluation of drugs. Relying on this center, the “Kunming Primate Research Center of the Chinese Academy of Sciences”, “Chinese Academy of Sciences and Yunnan Province Animal Model and Human Research Center”, and “Kunming Primate Research Center of the Chinese Academy of Sciences” were established. Key Laboratory of Disease Mechanisms”, “Experimental Animal Center of Kunming National Bioindustrial Base”, “Tree Shrew Breeding and Reproduction, Chinese Academy of Sciences Base” and the “National Kunming High-level Biosecurity Primate Experimental Centre”, which is under construction
1. Microbiological laboratories
2. Virus laboratory
3. Biochemical laboratory
4. Pathology laboratory
5. Autopsy room
And the number of experimental monkeys, more than 1,400, no need to marvel, has a strong strength, but this is not the most.
At the end of October 2004, the First International Symposium on Primate Biomedicine, hosted by the Kunming Institute of Zoology, Chinese Academy of Sciences, was held in Kunming. The grand event was quickly covered by Nature. Seeing the Institute’s primate experimental center with more than 1,400 experimental monkeys and strong scientific research, from Arizona, USA University neurophysiologist FraserWilson was so inspired that he decided to stay at the Kunming Zoological Institute for long-term research. In 2005, Prof. FraserWilson’s research project was funded by the Natural Science Foundation of China, which was also This is the first time that China’s Natural Science Foundation has given funding to a foreign scholar with a “high nose and blue eyes”.
It can be argued that the real owner of this research centre is the Academy of Military Medical Sciences, while the General Staff is in charge of security, and that in 2004, it was the General Directorate for Security. It was almost completed by the end of the year. This is in line with the takeover of the P4 lab that was built over 10 years later and everything is again amazing.
But it’s really just beginning. Let’s look at another story.
Establishment of an experimental platform for human diseases and non-human primates in Guangzhou
Article Source: Posted on 2004-08-06.
August 5, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences (CAS) and Guangdong Insect Research Institute (South China Institute of Endangered Animals) A collaborative project was signed in Guangzhou to build a platform for experiments on human diseases and non-human primates. In today’s society facing challenges such as AIDS, cancer, SARS, neurological diseases and cardiovascular diseases, primates are the most vulnerable species of human beings. Close relatives, which are highly evolved and have a high degree of similarity to humans in terms of tissue structure, immunity, physiology and metabolism, are in the They are irreplaceable experimental animals in life sciences and biomedical research and development, and their applications are far more valuable than those of other species. Last year, the CAS Guangzhou Institute of Biomedicine and Health, which was established by the Chinese Academy of Sciences, Guangdong Province and Guangzhou City, was established to address major medical issues. Applied basic and biomedical industry urgently needed core high technology research and development, through cooperation with medical centers or with biomedical enterprises. In order to promote the development of health medicine, the company has reached strategic alliances with other organizations and industries, which will have a leading and exemplary effect. and advances in the biomedical industry.
The Jiufo Base of Guangdong Insect Research Institute (South China Institute of Endangered Animals) under the Guangdong Academy of Sciences has 4,500 primates. Each year, it can provide more than 1,000 animals that meet international experimental quality standards to domestic and foreign experimental institutions, which is an important contribution to cooperation in life sciences and Research in comparative medicine, particularly in epidemiology, experimental animal models, disease mechanisms, drug screening and safety evaluation. It provides a good foundation for the development of biomedical research. In order to further promote the development of biomedical research in the country, the two sides, in the spirit of resource sharing, joint collaboration, mutual benefit and common development of the In principle, the two sides decided to cooperate to establish a non-human primate experiment at the Jiufo Base of Guangdong Insect Research Institute (South China Institute of Endangered Animals). The platform will be used as an important part of preclinical animal experiments for biomedical and health research in Guangzhou. The cooperation also includes: targeting the frontiers of life sciences and biomedical technologies to carry out research on major human diseases and infectious diseases. The original basic research on pathogenesis and infection mechanism has become an important support platform for China’s biomedical and public health research and development institutions. The research is aimed at the development of biomedicine through the study of human disease primate models and functional genes, with primate experimental animals as the target vector. Pharmacology and efficacy studies of technologies and products, etc.; establish technical support for preclinical safety evaluation of biomedical products and technologies. and experimental platforms, etc.
Note the phrase “The Guangdong Insect Research Institute (South China Institute of Endangered Animals) under the Guangdong Academy of Science has primates at the Jiufo Base. 4500 animals. Each year, more than 1,000 animals that meet international experimental quality standards can be supplied to domestic and foreign experimental institutions”. It is the base managed by the Guangdong Entomological Institute that has the true primate legion of laboratory animals. Of course you will find the following description in the official publication.
Center for Laboratory Animals, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, aims to provide high quality animal experimental services, adhering to the international standards. Medicine strict quality management of animal experiments and standard operating procedures for animal experiments, focus on animal welfare, innovative introduction of 6S management system. We strive to build a first-class international laboratory animal research base. It has passed the facility inspection by the Guangdong Provincial Science and Technology Department and obtained the license to use experimental animals. In addition, the center is developing in the direction of information management, and has established a database of rodent histological profiles, teratoma analysis profiles. Databases and an electronic information management system is being constructed.
There are three experimental facilities in the center, including: the general level large animal laboratory of 1,000 square meters, which can keep 96 experimental monkeys and more than 300 experimental rabbits; the SPF level animal laboratory of 1,500 square meters, which can keep more than 20,000 mice; the Jiufo non-human primate large animal laboratory of 320 square meters, which can keep 150 experimental monkeys; the aquatic animal laboratory of 200 square meters, which can keep 1,500 clawed toads and more than 1,600 zebrafish; and the P2 laboratory of 200 square meters, which can keep 3,000 mice.
In addition, the center is equipped with more than 20 million worth of instruments and equipment, including small animal live-imager, multi-conductor physiological recorder, small animal ultrasound imaging system, etc., which can basically meet the needs of animal models of stem cells, infectious diseases, metabolic diseases, tumor diseases, drug metabolism and histopathology research.
The center can provide standardized laboratory animal management, embryo purification and cryopreservation, model animal breeding and maintenance, animal model construction and evaluation, and animal model development. The center will introduce special strains, pathology experiments, training of laboratory personnel and animal experimental design consulting services. The Centre will explore a new path in the standardization of experimental animals and animal experiments that is suitable for China’s national conditions and in line with international practice. It provides a full range of experimental animal services to support the high level of biomedical research and domestic and international exchanges and cooperation of our institute.
It is clearly stated in this material that the Experimental Animal Center of the Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences has a Jiufo non-human laboratory. Three experimental facilities at the Primate Centre, including: a general grade large animal laboratory of 1,000 m² with a capacity of 96 experimental monkeys. More than 300 experimental rabbits; 1500m2 SPF-grade animal laboratory, which can feed more than 20,000 rats and mice; Nine Buddhas inhumane The primate large animal laboratory is 320m2 and can house 150 lab monkeys, for a total of 246 monkeys, so the remaining After SARS, the Chinese Academy of Sciences and the Guangdong Provincial Government collaborated to establish the Chinese Academy of Sciences in Guangzhou as a key project. Is the Biomedical and Health Research Institute just about managing 246 monkeys? The answer will be revealed in the final chapter.
Well, the monkeys have been found, we’re not going to do any more digging for other experimental animals because that’s no longer important, so if you need to, go check the public websites, go dig, and there may be more monkeys.
Part 3: Scientific and technical personnel with knowledge of recombinant viruses and cleansing technologies and hardware for research and experimentation with recombinant technologies
The great leader Chairman Mao once taught us, “A revolutionary team is the key!” So it’s not the 60,000+ strains of live viruses or the thousands of monkeys used for testing that are at the heart of this, it’s the people. In a previous article we have already revealed that the Chinese communist state uses blue and gold tactics to mine tech talent and steal tech technology from the US The truth of the matter is that its purpose is to 3F the United States, which means to weaken, confuse and destroy it. Since the viral genetic weapon is the weapon of mass destruction for the final battle, the team making this weapon must be secret and huge. However, the core is “technically sound”, that is, to ensure that after a decade or more of preparation, the country will be able to take the lead. This team is capable of accomplishing this task.
So let’s see if the core team assembled by the Wuhan Institute of Virology and the State Key Laboratory of Virology of the Wuhan University College of Biological Sciences can accomplish this task.
Wuhan Institute of Virology Team
The Wuhan Institute of Virology’s team profile we have already shown in the first season’s digging and blasting material, here is just one statistical statement.
Statistical table of personnel employed by the Wuhan Institute of Virology (not separately in this table as it appears in the table below).Statistical table of personnel employed PDF
Total 65 of whom 35 are Researchers, 25 are Associate Researchers and 5 are Young Researchers. Fifteen of them are shown to be working in the “State Key Laboratory of Virology”. Interestingly, Shi Zhengli, Yuan Zhiming and others who participated in virus isolation and looked for viruses in various animals are not in the State Key Laboratory of Virology. The lab. Among these 15 people, only Dunphy’s research is related to the search for the virus in the field. Therefore, the people who are doing the actual work on the surface do not have access to the core secrets.
“The team of the State Key Laboratory of Virology.Laboratory of Virology PDF
This table lists a total of 60 people, including 4 managers; 3 engineers; and 53 researchers. Fifteen of them work at the Wuhan Virus Institute.
The following table lists the mailing addresses
From the Charts above, we can conclude:
A. Nepotism/The relationship among these people – they are all related: either relatives or friends or families. Graduate students come from Wuhan U, Wuhan Virology Institute or the other schools from Wuhan.
B. Studying/working abroad experience – vast majority of these people had been abroad. Most of them went to the United States, followed by France, the Netherlands, Germany, Japan, Australia and Singapore
C. The address of these people’s correspondence – Xiaotangshan, Wuhan University Life Science Institute, Wuhan University School of Basic Medicine
D. Their domestic experience – The following units have emerged: Institute of Microbiology, Chinese Academy of Science; Institute of Biophysics, Chinese Academy of Science
These elements indicate a structure and relationship of personnel management in the “National Key Laboratory of Virology”. The laboratory is in the center, and its research relies on the three scientific research units: Wuhan University, Wuhan Institute of Virology, Wuhan University School of Basic Medicine. The lab’s personnel training, talents pool also relies mainly on these three units. The great thing about this structure is that it covers up the truth beautifully. Imagine, if Mr. GUO Wengui hadn’t told you about GUO Deyin, you wouldn’t have thought about the relationship between this lab, the P4 lab and the CCP virus. Such management structure is tantamount to putting a mask on the devilish practice of developing biological and chemical weapons, which is not only financially guaranteed at the national level, but also does not attract the attention of the international community and the United States and makes everything sounding reasonable and legal.
This management structure, of course, has more than one meaning 1) The National Key Laboratory of Virology is the central nerve system of the whole operation, it manages the research work of the subordinate units (of course, the core related to genetic weapons research), and at the same time tightly control the research progress and direction of the following three units, that is, assigning, directing and leading the tasks for which it is responsible, and the results of this process will certainly be reported to the academic committee, because it is responsible for only a core part of genetic weapons development, that is, the development of viral weapons, and the rest of the work needs another team to do it, so there must be a pipeline. 2) Completion of its milestones will inevitably lead to a strategic shift, that is, realignment of key personnel. At the same time, important resources are bound to be transferred. Of course, the institution will still be there for the other reason, but its core mission has changed. 3) This structure makes it possible for the critical research and development to be carried out not necessarily in this laboratory, but in the three dependent units where much of the basic work and preparatory work can be done; the virus synthesis part of the production must be done by the core trusted staff, while the experiments and tests can be concluded not in the dependent unit, but in a more clandestine team, of course, the dependent unit researchers can participate, and on the surface must be dependent on the Wuhan Virology Institute.
Because of this structure and management, some of the key characters involved in the biochemical weapon development, their names have not emerged. People such as SHI Zhengli, WANG Yangyi, and YUAN Zhiming, they are the frontline researchers who conduct basic ground works and they are not responsible for putting pieces together or communication among the units. Only in this way, it can guarantee the execution of the strict confidentiality system. Scientists such as SHI Zhengli etc. are unaware of the lab’s core mission. There are people who know the core mission in the “management team of the laboratory”, and there are people responsible for communications among the units in the “academic committee”.
A. Nepotism among these people is inevitable, since the structures established by such nepotism will be reliable and not prone to leaks. It also proves the importance and level of secrecy of this mission.
C. Addresses – Zhengdian (郑店) None of these people’s addresses are related to Zhengdian. It indicates on the one hand that they are covering up the relationship between this lab and P4 lab, and on the other hand, of course, it is more important that the P4 lab is the final recipient of their mission, and there is a handover relationship, so there is no need for the relevant people to appear, as evidenced by the number of virus strains, the completed virus weapons were handed over to the military. But the original synthesis of this weapon must have been in the P3 lab.
D. Institute of Microbiology, Institute of Biophysics, Shanghai Pasteur and the Academy of Military Medical Science
Chart: Reporting Lines of the Units
From the above organization charge, we created the forms below:
1、 Personnel’s Main Experiences
Wuhan Virology Research Lab Personnel Experience Chartorganization charge PDF
A. As can be seen from the tables above, 13 people joined the Wuhan Institute of Virology in 2003 (before the start of the P4 laboratory), 27 people joined the Institute in 2004-2010 during the first Academic Council, 13 people joined the Institute in 2011-2016, and only 4 people joined the Institute in 2017 during the third Academic Council. From their experience, you know that most of them were targeted trained students and were chosen to work at the Institute, and their study abroad and visiting scholarships costs were largely paid by the government. Why made such an arrangement? There is only one possibility: to ensure the stability of the research. Who has such a power? It’s the Academic Council that has such a power to move personnel to and from various units. Only people from the very top can have access to this kind of strategic planning and can actually execute it. It is not possible for China’s science and tech institutions to make this kind of arrangement. Personnel’s transfers and abroad arrangements are all part of this strategic plan, and that’s why the green light is given all the way, which is rare in China.
B. Let’s look through the tables and find out who was TIAN Bo’s student in addition to GUO Deyin, WU Jianguo, XIAO Gengfu and WANG Yangyi. WU Shuwen was TIAN Bo’s Student. WU Shuwen “2006.9-2012.6, Wuhan University, PhD, Mentor: Professor TIAN Bo” – so was he one of the three graduate students who offered services to SHU Hongbing, as GUO Deyin claimed? It is unknown.
C. From these tables we also know that there are 8 people who have studied in the Department of Virology at Wageningen University in the Netherlands, they are alumni, namely, WANG Manli, SUN Xiulian, HU Zhihong, DENG Fei, SHEN Zhu, WANG Hualin, PENG Ke and CHEN Xinwen.
D. YAN Bing’s resume is very simple and no other information can be found.
2. Analysis of Key Personnel’s Academic Papers
We have listed the scientific and technical papers published by people from the “National Key Laboratory of Virology”, including 15 people from Wuhan Virology Research Institute, and asked our experts to interpret them:
The essential elements of producing biochemical weapons are the separation of viruses from the wild animals, the modification of viruses, and the development of antidotes to the viruses. The number of people actually involved in the development of biological and chemical weapons and the number of people who know the secret is very small. The vast majority of people in the institutions are just ordinary scientists who are kidnapped by the Chinese Communist Party. But the findings of these scientists, their technology, and their viral strains used in their research have built a huge platform and are being used as resource reservoirs for CCP to develop biological weapons and drugs. CCP’s military takes the fruits of the scientists’ findings and their technology to use for evil purposes without their knowledge. We categorized these scientists’ research papers by the type of research, and marked their names with the relevant colors. We want people to understand CCP’s strategic plan through these research papers.
Use Red color for research which mainly involves in finding virus or bacteria from various animals (academically it’s called studying of virus or bacterial evolution-but it is used by the Chinese Communist Party as a biochemical weapons resource bank)
Use Blue color for research mainly involves in the study of the cellular and molecular mechanisms of viral infections or the development of new technologie development (the study of the basic mechanisms of viral infections is not directly related to the development of viruses and antidotes, but to some extent can serve as a theoretical reference and technical support for military bioweapons research)
Use Green color for research mainly involves in virus antidotes, i.e. small molecule inhibitors, peptide inhibitors and antibody vaccines (directly involved in the development of antidotes).
Use Orange color for research mainly involves in protein purification of viruses, biochemical or biophysical molecular interactions and things associated with biomaterials (which provide the molecular basis for the development of antidotes)
We can summarize this: majority of the scientists, for the most part, are normal scientists – their work mostly focus on molecular mechanisms and antiviral. But the entire output of these scientists is ready to be taken or used by CCP’s military for biological weapons research. These scientists’ research covers the molecular mechanisms of the various steps in the viral infection process, key protein interactions, small molecule inhibitors and various aspects of vaccine development. Various common viruses are also covered. And with the military having these resources, building biological weapons is as simple as baking bread.Papers PDF
The following is the official profile of the Wuhan Virus Institute (this profile was published in 2008 when Chen Xinwen was director, and was detailed and accurate):
Wuhan Institute of Virology, Chinese Academy of Sciences
The Wuhan Institute of Virology, Chinese Academy of Sciences is located on the shore of the beautiful East Lake in Wuhan. It was founded in 1956 and is a comprehensive research institution specializing in virology research. It was approved to become part of the Knowledge Innovation Project Sequence of the Chinese Academy of Sciences in 2002 and 2006 (Phases II and III). The focus of the discipline is expanded from the original general virology to the research of medical viruses and new viral diseases. The scientific and technological goals of Wuhan Virus Research Institute are: to meet the strategic needs of sustainable national agricultural development, population health and national security; to be at the forefront of international virology research; to focus on agricultural virus (agricultural microbe) and medical virus research; to strive for original cutting-edge scientific innovations; to develop important viral disease detection and epidemic prevention technologies through key technological inventions and integrations, and to make fundamental, strategic and forward-looking contributions to the sustainable development of Chinese agriculture, human health and national security.
The scientific research layout includes a molecular virology laboratory, a biological control laboratory, an analytical biotechnology laboratory, and a Chinese virus resource and information center. There are 21 research discipline groups in total. It boasts the State Key Laboratory of Virology (co-established with the Wuhan University), Sino-Dutch-French Invertebrate Virology Joint Open Laboratory, HIV Preliminary Screening Laboratory, Hubei Provincial Viral Disease Engineering Technology Research Center, and other technological research platforms such as the Chinese Viral Resources Scientific Database. The technical support system consists of a large-scale equipment analysis and testing center, a monoclonal antibody laboratory, an experimental animal center, the editorial department of <Virologica Sinica>, and a network information center. The management system consists of three functional departments: an integrated office, a human resources department, and a scientific research finance planning department.
China Virus Resource and Information Center has the largest virus depository in Asia, with over 900 strains of various viruses. This has established China’s only “National Viral Herbarium” with modern display, integrating academic discipline, special characteristics and scientific education. It is one of the first “National Youth Demonstration Bases for Science and Technology Activities in the World of Science”.
Over the past 50 years since the establishment of the Institute, through arduous and monumental efforts, generations of scientists have made important contributions in the development of virology discipline, the cultivation of academic talents, and the promotion of national society and economic development. It has obtained 227 scientific and technological achievements, 96 award-winning achievements, published more than 1,800 academic value papers and 50 advanced research monographs, applied for 119 patents, and authorized 73 patents. It has frequent academic exchanges abroad, with good cooperative relations with prestigious universities and research institutions in more than 30 countries and regions, including the United Kingdom, Netherlands, Germany, and World Health Organization. More than 20 well-known Chinese and foreign scholars and experts also serve as visiting scholars at the research institute. As one of the few Chinese institutions cultivating senior talents in virology, it began to enroll postgraduate students in 1978. There are currently 2 doctoral and 2 master degree granting points in microbiology, biochemistry and molecular biology. More than 300 postgraduates in virology-related majors have been trained, and more than 240 Ph.D. and master degree students are currently studying. The sponsored <Virologica Sinica> is the core periodical of Chinese biological medicine and the authoritative publication of virology. constantly featuring in American publications and databases such as BA and CA.
The Wuhan Institute of Virology has a high-level research team with a core of young scientist. There are 180 current staff, 152 professional technical personnel, 72 senior personnel, including 25 high-ranking. Of the researchers, 50% are under the age of 45. There are 82 scientific researchers, with a median age of 38, 83% of whom decorated with PhD and master degrees. It inducted 6 talents from the “Hundred Talents Program” of Chinese Academy of Sciences, 2 winners from the “The National Science Fund for Distinguished Young Scholars”, and 2 candidates from the first and second tiers of the National “Cross-century Project”. A group of ethical and talented disciplinary leaders are emerging and making their mark on the international academic stage.
The Institute has excellent facilities for scientific research. There are multiple types of advanced scientific research equipment with a value of over 21 million yuan. It has a BSL-3 laboratory and a 600 square meter SPF level animal laboratory. The Hubei Provincial Microbiological Society and Wuhan Microbiological Society are affiliated with the Institute.
The Wuhan Institute of Virology, through its modus operandi “truth and pragmatism, unity and cooperation, courageous innovation, and pursuit of excellence”, is creating scientific and technological glory for the motherland in a quiet, beautiful, and harmonious scientific research environment, and is striving to contribute to the development of virology in China and the national economy.
Director Chen Xinwen
Chen Xinwen, Wuhan Institute of Virology, Chinese Academy of Sciences, male, born in 1964, PhD, researcher. He received a bachelor’s degree in biology from Huazhong Normal University. He graduated from Henan University of Technology (formerly Zhengzhou Food College) in 1988 with a postgraduate degree in biochemistry. In 2001, he obtained a PhD degree in virology from Wageningen University in the Netherlands.
He went to the University of California Berkeley and Wageningen University Netherlands to engage in cooperative research. He was elected by the Hundred Talents Program of the Chinese Academy of Sciences, and a winner of the The National Science Fund for Distinguished Young Scholars. He holds the title of Director of the Wuhan Institute of Virology, Chinese Academy of Sciences, deputy director of the Academic Committee of the Wuhan Institute of Virology, member of the Academic Degree Committee of the Institute, director of the virology laboratory, and head of the Hepatitis Virus and Gene Therapy Discipline Group. He is also the editor-in-chief of <Virologica Sinica> and a member of the editorial board of <China Biological Abstracts>. He is a consultant of the Virology Committee of the Chinese Society of Microbiology, executive director of the Hubei Provincial Microbiology Society, and executive director of the Hubei Bioengineering Society. He has been long engaged in basic research of molecular virology, and has conducted in-depth research on the structure and function of the baculovirus genome, the interaction between the virus and the host, and the genetic function of the hepatitis virus. Currently, he is undertaking research projects such as “973” and the National Natural Science Foundation of China. He published more than 70 research papers in core journals at home and abroad, including more than 30 SCI source journal papers; participated in the preparation of two advanced research monographs. He won the second prize of National Science and Technology Invention Award, the second prize of Hubei Province Natural Science, the outstanding graduate research supervisor of the Chinese Academy of Sciences and the fifth Wuhan Excellent Youth Award amongst other honors and awards.
1. Academic Committee of Wuhan Institute of Virology, Chinese Academy of Sciences
Director: Yuan Zhiming
Deputy Director: Chen Xinwen
Committee members: Hu Zhihong, Zhang Xian’en, Shi Zhengli, Wang Hanzhong, Chen Ze, Chen Shiyun, Li Tianxian, Peng Huiyin, Zhou Yafeng, Zhou Ningyi, Gao Shoujiang
Secretary: Liang Li
2、Degree Committee of Wuhan Institute of Virology, Chinese Academy of Sciences
Director: Chen Shiyun
Deputy Director: Shi Zhengli
Committee Members: Chen Xinwen, Chen Ze, Hu Zhihong, Li Tianxian, Peng Huiyin, Wei Hongping, Wang Hualin, Wang Hanzhong, Yang Rongge, Yuan Zhiming, Zhou Ningyi
3、Biosafety Committee of Wuhan Institute of Virology, Chinese Academy of Sciences
Director: Yuan Zhiming
Deputy Director: Chen Ze, Chen Shiyun
Committee Member: Hu Zhihong, Gong Hanzhou, Chen Xinwen, Li Tianxian, Song Donglin, Zhang Zhiping, Wang Hanzhong, Yuan Li, Sun Xiulian, An Xuefang
Secretary: Song Donglin
State Key Laboratory of Virology
The State Key Laboratory of Virology was approved for construction in 2004. It is one of the important bases for China to carry out basic theoretical and applied virology research, cultivate high-level talents in virology, and promote international and domestic scientific research cooperation and academic exchanges in virology and related fields.
Academician Tian Bo, Honorary Director of the Academic Committee of the Laboratory, Academician Chen Huanchun, Director of the Academic Committee, and Wu Jianguo, Distinguished Professor of the Yangtze River Scholars, Director of the Laboratory. Based on scientific development trends, national needs and human health needs, the laboratory aims at the frontiers of virology research, takes viruses that cause major and sudden diseases as the main research objects, and uses virology and modern biotechnology to study the molecular process of virus infection and its accompanying life phenomena at different levels, from viruses, host cells, the body to a population; to reveal the molecular mechanism of virus immunity, pathogenicity, and carcinogenesis and the epidemiology of viral diseases; to improve our virus resources and information database; to develop new technology for viral disease diagnosis, prevention and treatment. The current main research directions of the laboratory are: viral genetic variation and molecular epidemiology, molecular mechanism of virus-cell interaction, viral immunity and pathogenic mechanism, and basic research on the prevention and treatment of viral diseases.
The laboratory currently has a relatively high-level research team, with 40 permanent staff and 20 mobile staff. Among them, there are 1 academician of the Chinese Academy of Sciences, 1 distinguished professor of the “Yangtze River Scholar” of the Ministry of Education, 4 winners of the “Hundred Talents Program” of the Chinese Academy of Sciences, 4 winners of the National “Funds for Distinguished Young Scientists”, 3 “Cross-Century Talents” of the Ministry of Education, 2 “New Century Talents” of the Ministry of Education, 1 “Luojia Scholar” Distinguished Professor of Wuhan University, 1 “Barry and Martin’s Prize” winner, and 3 “Hubei Province Funds for Distinguished Young Scientists” winners. The laboratory has undertaken a number of major and key national scientific research projects. It has formed the characteristics and advantages of combining the basic theory and application of virology. In SARS virus and bird flu virus animal traceability and molecular epidemiology, insect virus infection and biology Prevention, important human virus infection, immunity and pathogenic mechanisms, virology research new technologies and methods the laboratory has achieved some high-level research results, some of which have had important impacts at home and abroad
At present, the laboratory has a utilization area of 7000 square meters and nearly 100 pieces of various instruments and equipment. It has a level-three biological safety (BSL-3) laboratory, an animal level-three biological safety (ABSL-3) laboratory, national typical culture depository, national virus resource and information center and advanced scientific research conditions and technical platforms such as SPF-level Laboratory Animal Center and AIDS Center. These meet the needs of carrying out basic theoretical and applied basic research in virology and related fields. (This also proves our analysis of the management structure above)
Laboratory of Molecular Virology
Viral Functional Genomics and Genetic Engineering Discipline Group (Leader: Hu Zhihong)
Its predecessor was the Institute ’s key discipline of insect virus molecular biology. After strategic structural realignment, the research field has been expanded to include virology basic and applied research including various emerging viral diseases. The key directions include viral functional genomics, genetic engineering technology to improve recombinant virus insecticides, molecular biology and epidemiology of novel viral diseases, analysis of viral infection mechanism and screening of antiviral drugs. Current research subjects include baculovirus, SARS coronavirus and hepatitis B virus. It has undertaken a number of projects of 973, 863, National Funds for Distinguished Young Scientists, National Natural Science Foundation, European Union’s sixth framework project and the China-Netherlands Science and Technology Strategic Alliance Plan. It has long-term cooperation with advanced laboratories in countries such as the Netherlands, Germany, and Canada.
Hepatitis virus molecular biology and gene therapy discipline group (leader: Chen Xinwen)
To carry out molecular epidemiology, molecular biology, drug resistance mechanism, virus and host interaction and other research of hepatitis B virus (HBV) and hepatitis C virus (HCV), important viral diseases that endanger human health, such as hepatitis B and hepatitis C, to reveal the molecular mechanism of virus pathogenicity, develop genetic engineering vaccines, and explore new diagnostic and therapeutic methods (gene therapy).
To study the mechanism of baculovirus invasion into mammalian cells, the state of baculovirus genomic DNA in mammalian cells and experimental animals, and the expression of target genes. To understand the safety and efficacy of baculovirus for gene therapy and basis of application. At the same time, a series of baculovirus vector systems with independent intellectual property rights will be constructed and used for gene therapy of important diseases.
Influenza Virus Discipline Group (Leader: Chen Ze)
The main research directions are: the application of human influenza virus nucleic acid vaccine and its basic research, including DNA vaccines, recombinant vaccines, subunit vaccines, inactivated vaccines, mucosal vaccines and vaccine adjuvants; avian influenza virus epidemiological investigation and influenza virus cross-species (family) infection mechanism research. To further understand the composition of influenza virus storage source and animal chain, to clarify the relationship between its genetic variation and virus pathogenicity; SARS virus vaccine research and therapeutic drug development. At present, the projects undertaken include the “Hundred Talents Plan” project, a national “863” research project, a national major scientific and technological research project, an innovation direction project of the Institute, two projects supported by the National Natural Science Foundation of China, the Provincial Science and Technology Department and the Education Department SAPS research special fund. More than 30 papers have been published.
Aquatic Virus Discipline Group (Leader: Shi Zhengli)
Engaged in the research of fish, shrimp, crab and shellfish and other major economic aquatic animal viruses and algae viruses. Including the isolation, identification and classification of viral pathogens, the study of the genome and protein functions of important viruses, the molecular mechanism and immune mechanism of viral pathogens, and the establishment of virus diagnostic technology. At present, it has presided over the National 863 Marine Biotechnology Projects, the Chinese Academy of Sciences Knowledge Innovation Key Directional Subprojects and the National Natural Science Foundation projects.
Zoonosis Discipline Group (Leader: Wang Hanzhong)
Mainly involves the research of several important zoonotic viruses, such as SARS-CoV, foot-and-mouth disease virus, rotavirus of humans and animals, and other new disease pathogens. The current research focus is on the natural host and origin of SARS-CoV virus Research and the molecular evolution and molecular epidemiology of SARS-CoV virus, foot-and-mouth disease virus and rotavirus, and the interaction between virus and host, revealing the molecular mechanism of virus pathogenicity, the principle of virus-host interaction, virus toxicity， identification and functional analysis of viral genes, the development of viral vaccines, and the development of rapid and sensitive early diagnostic reagents, providing a theoretical basis for the prevention, early warning and forecasting systems of major zoonotic and novel viral diseases in China.
Oncological Virology Discipline Group (Leader: Gao Shoujiang)
The long-term research goal is to explore the pathogenic mechanism of human tumor diseases caused by viruses, especially the epidemiology and related analysis of tumor diseases in China, and to reveal the molecular mechanism of virus-induced malignant transformation. The research results will provide a scientific basis for the exploration of prevention and treatment of viral induced diseases such as cancer. The discipline group maintains a close cooperative relationship with the University of Texas Health Science Center (Tumor Virology Program in the University of Texas Health Science Center at San Antonio).
Discipline Group of Molecular Biology Research on HIV Virus (Leader: Yang Rongge)
Mainly devoted to the molecular biology research of AIDS and HIV virus. Including HIV infection mechanism, mutation and epidemiology. Recombination of HIV virus, the role of HIV virus and human immune system. These studies are based on genetic and cellular levels. Based on the research results of HIV, develop new HIV vaccines. All these studies will provide theoretical and technical support to improve the control and prevention of AIDS virus in China.
Discipline group of virus replication and antiviral drugs: (Leader: Chen Xulin)
The main research direction is based on understanding the detailed mechanisms of major viruses from adsorption invasion, transcription and replication of viral genomes, virus assembly and maturation release, the establishment of models screening drug target sites of human immunodeficiency virus (HIV), hepatitis B virus (HBV), avian Influenza virus (AIV), Herpesvirus (Herpesvirus), screening for high efficiency and low toxicity antiviral drug from chemical compound libraries from different sources (Chinese herbal medicine, marine drugs, microbial metabolites, artificially synthesized small molecules, etc.)
As our scientist explained above, all research institutes, schools, researchers, professors, students trained, as well as viral resources and laboratory animals are regarded as the party ’s private property. The wealth of knowledge and technology acquired by Chinese people around the world through diligence and intellect is completely stolen and hijacked by this organization. These laboratories, research institutes, and senior scientific and technical personnel consciously and unconsciously became accomplices in the development of biological and chemical weapons. The hijacked ability of Chinese biomedical research, covet from the world forms the entire foundation and soft power of CCP’s bioweapon development. This ability makes it really easy to develop a SARS-like biochemical weapon. Of course, CCP definitely will not develop only one virus.
Part 4: Key Step: Control and use of viral weapons
In previous articles, we have outlined the process of developing a biochemical virus weapon and what it takes. This weapon must be to be controlled. The actual effectiveness and importance of a biochemical virus weapon has been outlined in the book “Unrestricted Warfare”. So this viral weapon must be highly diffusible, transmissible through aerosol (the main way coronavirus is spread), and low lethal. The core is to create a panic, not killing. The virus weapon must be controllable, which means it cannot be released to kill CCP itself. The only way to call it a weapon is to be able to put it out against the enemy.
Then the weapons control section would cover two areas: A. How to control B. How to predict and test the effectiveness of the weapon in killing the enemy. This control solves a problem not fundamentally different from the development of conventional weapons, but of course the process is not unlike normal medical control of viruses The development of a vaccine (a weapon against a virus) is essentially the same. Specifically, A, to develop something that suppresses the virus or is capable of fighting it. B, to test a viral weapon (note that not experiments) and develop transmission control models to derive the effect of virus use. Let’s start with B: Unlike normal vaccine development, the process of such testing must include testing of transmission routes and methods of transmission. experimentation. The later stages of vaccine development are experiments and do not include testing of transmission routes or methods of transmission. In other words, once the natural route of transmission of a virus to a human is identified and found, it does not need to be tested to prove it, and man-made viral weapons are not a part of the test. The test is only needed because the virus does not exist in nature and the effect and route of transmission is unknown. Experiment by experiment (laboratory) and trial by trial (simulated natural environment) are necessary to find and confirm the best methods and pathways of communication to people.
So, experiment and test is sequential, first in the lab, to determine the ability to infect lab animals from low to high”. The “guinea pig”, the “experimental pig” and finally the “primate” monkey. After determining the effect, test the “monkeys” and “pigs” in a field environment, and the final weapon must be the same as SARS in the population. Testing. In testing, large amounts of data are collected that are quite important for modeling control. The mathematical model of viral weapon control can be understood as a large-scale mathematical calculation, similar to the one now used to simulate thermonuclear weapons in the laboratory. As with the tests, it is no longer necessary to actually detonate a hydrogen or atomic bomb in the area, although the establishment of data from these tests is a fundamental step. So it’s not surprising that military personnel appeared in the SARS and 2016 swine fever outbreaks, their real purpose is not epidemic prevention, that’s a matter for the government department CDC, the real purpose of these military personnel and CAS research units appearing is to collect virus samples and data on the spread of the virus, which is the basis of all research.
A. The control of viral weapons, consisting mainly of 1 Inhibitor 2 vaccines 3 medical supplies for prevention and control, where the inhibitors are the virus inhibitors, generally refers to small molecule drugs, but can also be a drug like hydroxychloroquine, whose main role is to suppress the symptoms brought on by the virus. As we learned in the previous analysis of the difference between viruses and bacteria, the ultimate solution against viruses is a vaccine, and inhibitors are simply suppressors. That is to say, if you are infected with the virus, medication can suppress the symptoms of the virus, but it still depends on your immunity, so an inhibitor is not an antidote. Only a vaccine is the real antidote. The reason why medical supplies are included as part of the control of viral weapons is because the only way to avoid being infected with the virus without an antidote or suppressant is to physically isolate it using medical virus control equipment and methods. Imagine what the release of a viral weapon would do to an infected country and its people if the CCP had control of the inhibitors, the antidote, the vaccine, the masks for physical isolation, the gloves and the respirators for treatment. Meaning that your life or death is in their hands, which is the true meaning of Community of shared future for mankind what CCP is advocating, that the fate of humanity is in their hands
Let’s summarize the development process of viral biological weapons in one table.
The process of development and use of biochemical virus weapons
This is the entire process of development, production and use of viral weapons. In this process, the mathematical model of virus control, the mass production of viruses, and the use of viral weapons must be the most confidential sectors. because the most critical parts of the viral genetic weapon are here.
The process of developing a viral weapon is essentially not that different from the study of viruses and the Academy of Sciences study of viruses to destroy and defeat them, but at the heart of it is who controls the direction of that research. So it makes sense that Shi Zhengli said that the Wuhan virus is not produced in her laboratory, because scientists like Shi Zhengli only do research for money, obeying the organization’s orders, and benefits, and they can not control the direction of research. Even so, it is necessary to fabricate lies to cover up the truth about the development of biological and chemical weapons, because it is too big, it is a challenge to the whole humanity, it is a challenge to the United States.
It was based on this simple logic analysis that DT dug up the P3 lab as the place where the viral weapon was actually created. When this weapon was developed by two teams from the Wuhan University Institute of Biological Sciences and the Wuhan Institute of Virus Research after scientific efforts and a swine fever trial in 2016, the method and recipe for virus production (isolated live strains) was then handed over to the military. While key personnel completed a kind of strategic shift, the P4 lab was activated for mass production and stockpiling of viral weapons. It doesn’t matter where the virus is made, because there are too many P3 labs that can make the virus based on an established technical solution.
The virus control part, as we can see in the table above, encompasses both vaccines and inhibitors. As we have seen above, the development of inhibitors must be highly correlated with the 500,000 sample small molecule compound library donated to the Shanghai Institute of Pharmaceutical Sciences, Chinese Academy of Sciences, by the Institute of Biophysics, Rao Zihe and Novo Nordisk in early 2007. In addition, considering the high degree of coincidence of 2016 swine fever trial and two appointments (Wang Yangyi as Deputy Director of the Institute of Viruses and Tang Hong as Director of Shanghai Pasteur) all of this digging is telling us that we are very close to the real big bosses and manipulators behind the scenes!
Since this controlled study is first and foremost about inhibitors, let’s start with inhibitors.
Let’s read a post first:
Excerpts are as follows
Tsinghua University and other teams find answers in bats that are “invulnerable”: new coronavirus inhibitor found
Bats are “invulnerable” to natural reservoirs of viruses, so why are they immune to carrying so many viruses? Can mankind seek a universal idea for dealing with multiple viruses from here?
On March 31, Beijing time, the Center for High Precision Innovation in Structural Biology at Tsinghua University, Duke National University School of Medicine, China CDC, CAS Animal Research Institute, and Duke University jointly pre printed the paper on the website BioRxiv, “Orthogonal genome-wide Screenings in bat cells identify MTHFD1 as a target of broad antiviral therapy”. The study has not been peer-reviewed.
Through a systematic and comprehensive screen of more than 20,000 genes in bat cells, the team identified dozens of key bat genes on which viral replication depends and discovered a common new host gene, MTHFD1.
Further research revealed that bat cells express MTHFD1 at much lower levels than cells from the corresponding human tissues, which may be related to the physiological changes that bats undergo to adapt to life in flight.
The team eventually found that the inhibitor of the host protein MTHFD1, carolacton, effectively suppressed coronavirus replication.
They believe that the research results will not only support the development of new coronavirus drugs to combat the epidemic, but also lay the foundation for the future fight against virus outbreaks in humans.
Professor Xu Tan from the Center for Innovation in Structural Biology at Tsinghua University and Professor Linfa Wang from the Duke National University of Singapore School of Medicine contributed to the paper. The co-corresponding authors. Jin Cui, Ph.D. student, from School of Pharmacy, Tsinghua University, Qian Ye, postdoctoral fellow, and Daniellefrom Duke-National University of Singapore School of Medicine Anderson, and Dr. Baoying Huang from the China Center for Disease Control and Prevention (CDC) were co-authors of the paper. The study was also supported by researcher Wenjie Tan from the Chinese Center for Disease Control and Prevention, researcher Xuming Zhou from the Institute of Zoology, Chinese Academy of Sciences, and Professor So Young Kim from Duke University.
In addition, this work in Tan Xu’s lab is jointly funded by Tsinghua University’s epidemic prevention and control Science and Technology Tackling Emergency Special Project, the Beijing Center for High and Precision Innovation in Structural Biology, the Tsinghua-Peking University Joint Center for Life Sciences and the National Natural Science Foundation of China’s Outstanding Youth Fund.
Humans need broad-spectrum antiviral drugs
From SARS and Ebola to the new coronary pneumonia (COVID-19) in 2019, infectious diseases caused by viruses have been one of the major diseases that pose a serious threat to global health. The history of these epidemics reinforces the urgent need for research and development of broad-spectrum antiviral drugs.
Broad-spectrum antivirals can be invaluable in reducing mortality and mitigating epidemics by saving critically ill patients from acute treatment of new outbreaks of viral infections.
However, traditional antivirals target viral proteins, which are difficult to use in response to the many different classes of viruses that continue to emerge, and viruses can easily become resistant to drugs by mutating their genes.
In contrast, since many viruses require many common host proteins to complete their replication cycle in cells, novel antiviral drugs that target viral replication-dependent host proteins may have the advantage of being broad-spectrum and less susceptible to drug resistance.
The paper mentions that bats are a major natural host of the Ebola virus, SARS-CoV, MERS-CoV, the genus Henneba virus, and the new coronavirus. The research team sought to start with bat genome analysis and use leading functional genomics approaches to systematically search for virus-host factors of viral life cycles. New antiviral drug targets are sought by understanding the molecular interaction mechanisms.
Why are bats “invulnerable”?
Bats belong to the order Pteropodidae, the only mammals capable of true flight. In recent years, bats have been linked to a number of mass fatal epidemics, and they have been recognized as one of the most important natural “reservoirs” for emerging viruses.
The authors mentioned SARS in 2003, Ebola in 2014, and the coronavirus that began its outbreak in late 2019. Evidence supports that bats are a common natural host for these disease-causing viruses, and that transmission of the virus from bats to an intermediate host ultimately leads to the development of a new massive outbreak of the epidemic.
What is puzzling, however, is that while bats can carry a variety of disease-causing viruses, these viruses do not cause significant Symptoms. The bats’ high tolerance to viruses may also be an important reason for their ability to carry and transmit multiple viruses.
Zhou Peng, a researcher at the Wuhan Institute of Virology, Chinese Academy of Sciences, said in an interview with the reporter (www.thepaper.cn), “From an immunological point of view, the bat’s immune system is still very unique. It is the only mammal capable of flying continuously. The ability to fly causes many of its genes to be different from those of humans or other mammals. Many of these different genes are related to anti-virus and immune systems.”
Zhou Peng and others have previously confirmed that bats always maintain a certain amount of interferon expression. Interferon is a very important antiviral protein. If it is always kept “low” in the body, it is equivalent to the animal’s own “all-weather protection” defense mechanism.
“Our preliminary conclusion now is that its immune pathway will maintain a certain amount of defensive status, but it will not be over-immunized. Different from humans infected with SARS will eventually die from excessive inflammation, the inflammation and innate immunity of bats will not over react, so it will not be damaged.”
A similar thinking has been previously mentioned by Peng Zhou and others, and studying the uniqueness of bats carrying viruses without getting sick will hopefully allow humans to learn how to fight viruses.
The authors suggest that physiological studies and genome sequencing results in bats offer multiple explanations for their ability to tolerate viruses, and that functional genomics screens could help us further understand the host factors required for viral infection of bat cells.
More than 20,000 genetic screens: how does the mechanism of viral infection in bats differ from that in humans?
Based on the above background, the research team established the first whole-genome CRISPR knockdown library of bats (Pteropus alecto) and completed a whole-genome CRISPR screen for influenza virus infection in Pteropus alecto renal epithelial cells (PaKi cells), from which more than 20 host factors viral replication dependent on were found (Figure 1).
Meanwhile, Wang Linfa’s group at Duke National University School of Medicine in Singapore used RNA interference (RNAi) to screen bat cells for mumps virus infection and found dozens of virus-dependent host factors.
By comparing the results of the two screens, the team found that they all included important genes for endocytosis and protein secretion pathways, which are similar to those in human cells, suggesting that the dependence of viral infection on these pathways is conserved in both bat and human cells.
In addition, both screens identified a common new host gene, MTHFD1. MTHFD1 encodes methylene Tetrahydrofolate dehydrogenase, an important metabolic enzyme for the de novo synthesis of purine bases, the building blocks of DNA and RNA.
It is very interesting to note that none of the previous work on genome-wide screening of human cells for viral host factors found MTHFD1. Further studies revealed that bat cells have much lower levels of MTHFD1 expression than cells from the corresponding tissues in humans, which may be related to the fact that Physiological changes associated with bats adapting to life in flight.
Overall, the team conducted a systematic and comprehensive screen of more than 20,000 genes in bat cells and identified dozens of viral replication The key bat genes on which it depends. These genes are functionally conserved in bats and humans, but species differences in gene expression levels may determine differences in viral infection. The pathological findings.
Discovery of the inhibitor carolacton
The team further found that RNA viruses, including mumps virus, Maltavirus, and Zika virus, are responsible for MTHFD1’s The inhibitor of MTHFD1, carolacton, is very sensitive to the replication of these viruses. Inhibitory effect. This phenomenon is significant in both bat and human cells.
Carolacton is a natural product that has been used as an antibiotic candidate molecule to inhibit bacterial membrane production.
Encouragingly, in collaboration with the CDC, the research team found that carolacton also effectively inhibited neo-coronavirus replication in human cells, and the effective antiviral concentration was well below the cytotoxic concentration, demonstrating good drug-forming properties.
The team further found that RNA viruses, including mumps virus, Maltavirus, and Zika virus, are responsible for MTHFD1’s The inhibitor of MTHFD1, carolacton, is very sensitive to the replication of these viruses. Inhibitory effect. This phenomenon is significant in both bat and human cells.
Carolacton is a natural product that has been used as an antibiotic candidate molecule to inhibit bacterial membrane production.
Encouragingly, in collaboration with the CDC, the research team found that carolacton also effectively inhibited neo-coronavirus replication in human cells, and the effective antiviral concentration was well below the cytotoxic concentration, demonstrating good drug-forming properties.
In addition, the genome-wide screening system that the research team has established upfront will also play a huge role in targeting other tissue cells of bats, especially immune cells, helping researchers continue to probe more of the mysteries of bats.
Note that this article was published on March 31, 2020, long after the global outbreak, and that the article’s related authors, researchers and units are all caught up in the vortex of where the virus came from. Before we ask our scientists to interpret this article, let’s imagine how an ordinary person will feel after reading the article: the virus is no longer scary, a cure has been found. The team, who SARS, swine fever, and the new coronavirus originate from bats, found an antidote to coronavirus. The antidote is an inhibitor, which is in line with the traditional Chinese cultural concept of “within three steps there is an antidote”. “The Pill Valley philosophy thought up – the antidote is to be found in the animal host of the virus, the bat. This logical idea is tremendously confusing to an ordinary.
So how do scientists interpret this: A. Not sure about the correctness of this paper, the science section of this article states that First, the virus needs a host cell protein to replicate, and research has shown that this MTHFD1 is the host that the virus needs to replicate. The protein, which is abundant in humans and scarce in bats, makes it difficult for the virus to replicate in bats, so bats carry the virus but don’t get sick. In short, MTHFD1 was targeted and carolacton was later found to act as a viral inhibitor. B. Inhibitors are not vaccines, they are not antidotes, and they will inhibit the symptoms of infectious post-viral disease, and they will certainly be useful for treatment, but they can only reduce symptoms and suppress the virus, without vaccination. The only thing that can ultimately clear the virus is one’s own immunity, in other words, the effect of this drug on people with different immune systems. C. carolacton is an inhibitor and is a long way from being produced as a synthetic drug. There’s still a long way to go, as it has to go through clinical trials. All we can say is that it provides a technical direction.
Then the purpose of the article is very clear, it is published to the scientifically illiterate public. But it also exposes many important people and institutions to the public.
Key personnel and units related to the paper
Let’s start with a look at the biography of one of the key players Wang Linfa, who has already been profiled in an earlier article.
In 1978, he entered the Department of Biology, East China Normal University, Shanghai (now the College of Life Sciences, East China Normal University).
In 1982, he went to the University of California, Davis, USA to study abroad and received his PhD degree in biochemistry and molecular chemistry in 1986.
D. degree in biochemistry and molecular chemistry in 1986. In 1989, he obtained a postdoctoral degree and went to Australia in the same year, mainly engaged in gene cloning, molecular diagnostics, antibody genetic engineering and other aspects of research.
In May 1989, he went to Australia to work in the Department of Chemistry at Monash University.
In 1990, Linfa Wang worked at the Australian Animal Health Research Institute (AAHL) of the Commonwealth Scientific and Industrial Organization (CSIRO).
In 1995, he was selected as a CSIRO Outstanding Young Scientist.
In 1996, he was nominated for the “Australian Outstanding Chinese in Science and Engineering” award, and in the same year, he became the youngest member of the Australian Animal Health Research Institute (AAHL). Principal Investigator and Subject Director. Former Senior Principal Scientist and CEO Science Lead for the Federal Science and Engineering Organization (FESCO). In 1999, on the occasion of the fiftieth anniversary of the National Day, Mr. Wang Linfa was invited to join the “Hundred Doctors Tour”.
In 1999, on the occasion of the 50th anniversary of the National Day, Mr. Wang Linfa was invited to participate in the “Hundred Doctors Tour” and entered the Great Hall of the People four times and Tiananmen Square twice to participate in the ceremony.
In 2003, when SARS ravaged China and other countries and regions in the world, he was invited by the World Health Organization to visit China in August. Examining SARS disease prevention efforts. 
In 2010, he was elected as a Fellow of the Australian Academy of Technological Sciences and Engineering.
Over the past decade, Dr. Linfa Wang has made world-renowned achievements in the field of neonatal viruses and nascent infectious diseases, in particular He is a world leader in the study of Hendra virus, Nipah virus and SARS virus. In the last 10 years, he has published high level papers in journals such as Nature, Science, PNAS 10 As a member of the WHO expert group, he returned to China to carry out “Traceability of SARS virus”. ” studied tour and met with the leaders of the Ministry of Health, Ministry of Science and Technology, Ministry of Agriculture and the People’s Government of Guangdong Province. He worked on SARS traceability, tracing the relationship of Hendra and Nipah viruses to animals and humans, bat molecular immunology, etc. Wang Linfa has been a global leader in scientific research.
In the 2001 Shanghai Yearbook “Overseas Shanghai People”, Wang Linfa was listed among the ten overseas Chinese and Chinese who have made outstanding achievements and positive contributions to the motherland.
In 2000, Wang Linfa was appointed as a director of Shanghai Overseas Friendship Association.
An interview with Wang Linfa published on ScienceNet.cn on February 2, 2020 also caught our attention:
Public opinions are crazy! We cannot rely solely on CDC! Singaporean Professor Wang Lin’s Interview
In the early morning of January 31, the World Health Organization (WHO) decided to recognize the outbreak of novel coronavirus (2019-nCoV) infection as a Public Health Emergency of International Concern (PHEIC).
At the same time, the WHO highly praised China’s control of outbreak. But in China, as the “dumping” drama continues to unfold, various questions and controversies have been raised.
Also on January 31, Singapore announced the successful replication of 2019-nCoV, becoming the third country to achieve this result after China and Australia.
The study was led by Wang Linfa, a professor at the Duke-National University of Singapore School of Medicine and a member of the Australian Academy of Technological Sciences and Engineering who worked on the Chinese SARS study.
“Public opinions in China are crazy,” On January 31, Wang Linfa, who said he was “extremely busy,” pointed out in an interview with China Science News that when infectious disease outbreaks occur. Instead of relying solely on China’s Centers for Disease Control (CDC), the government should immediately form an “emergency national team”.
China Science News: The World Health Organization (WHO) has spoken highly of China’s control of the outbreak, but there have been some doubts in China. What is your assessment of the response to the outbreak?
● Wang Linfa.
Compared to SARS, the response to this outbreak was clearly better. The response was timely and the level of information disclosure was high, especially after the confirmation of “human-to-human” transmission. However, January 21 was a watershed event, and the performance up to that point was questionable.
Although public opinions are very crazy now, I think Chinese scientists have done very well in this epidemic, and the first discovery was made by clinicians.
SCIENCE CHINA: Was there a contradiction between the control of the epidemic and the scientific work and publication of papers during this period?
● Wang Linfa.
A bit of “competition” in scientific research is normal, and so is the case in other countries. I think the biggest lesson to be learned this time is to strengthen the collaboration among different government departments, instead of relying solely on CDC, because China. Most scientists are outside the CDC system.
For example, I work on bats and bat viruses, and I have a long and deep collaboration with the Wuhan Institute of Virology at the Chinese Academy of Sciences, which is, in my opinion, the world’s top institute for bat virus research, but also outside the CDC system.
In the event of an outbreak of infectious disease, the government should immediately organize a “national emergency response team” so that other institutions can work on an equal footing with the CDC. Participation and collaboration. If well controlled, the outbreak response and research should reinforce each other so that research during the outbreak response is active and meaningful The.
At the same time I strongly disagree with the personal attacks on any individual scientist, which are completely counterproductive. I would like to emphasize that this is an issue of a national emergency response system, not a personal issue or responsibility.
SCIENCE CHINA: Singapore also has a neo-coronavirus outbreak, what is the situation now?
● Wang Linfa.
The first confirmed case of novel pneumonia in Singapore occurred 1 week ago, so the successful replication of the novel pneumonia strain is a critical step in Singapore’s response to the evolving epidemic.
Our study was conducted in close collaboration with the Central Hospital, SingHealth-affiliated medical centres, the National Centre for Infectious Diseases (NCID) and the Ministry of Health to replicate 2019-nCoV from clinical samples from infected patients.
Our collaboration has been transparent from the outset and has already yielded considerable benefits.
China Science News: how do you assess the complete halt in SARS virus research after the 2004 laboratory leak? After SARS, China set up a direct reporting system for surveillance information that focused on monitoring all types of infectious diseases, but it doesn’t seem to be working this time?
● Wang Linfa.
It is true that in 2003-2004, three laboratories had SARS virus leaks, which caused some harm. However, in general, I think the ban on SARS virus research since then is an overreaction.
However, the monitoring and reporting system set up after SARS in China is still very useful, such as its role in the avian flu outbreak. important role, and I think it played a role in this epidemic as well. The main problem this time was that timely and effective action was not taken with the limited information available at the time.
China Science News: You have in-depth and ongoing research on bats and were involved in the SARS epidemic, do you think the virus came from bats?
● Wang Linfa.
Compared with 17 years ago, China’s scientific research capabilities have greatly improved, so this time I was not invited to participate in China’s new coronavirus research. But I personally think that this time the new coronavirus most likely still comes from bats. Now we just have to wait patiently for the results.
In 2005, I published a paper with Wuhan Institute of Virus Researcher Shi Zhengli and others in the journal Science, and the main conclusion was that the gene sequence analysis showed that the homology between the bat SARS-like virus and human SARS virus genome sequence was 92%.
However, it was not until eight years later that Shi Zhengli’s team isolated a strain of live virus similar to SARS virus from the dung of a chrysanthemum-headed bat in a bat cave in Yunnan province, with a high degree of homology to the known SARS virus, which clearly revealed the origin of the SARS virus.
It’s similar this time as well, and Shi Zhengli’s team has now found a bat gene sequence that has 96% consistency. I want to tell everyone, scientists and the general public alike, please be patient, scientists will eventually be able to confirm its origin, I It is considered highly likely that bats are still bats.
In fact, bats and viruses have a very symbiotic relationship that has existed for millions of years, long before the birth of mankind exist. And, our recent research confirms that bats are amazing colony mammals and that humans can learn a lot from them! I have a Singapore government-funded study called “Learning from bats: from genomics to viral control to fighting cancer”.
I have a Singapore government-funded study called “Learning from bats: from genomics to virus control to fighting cancer”. The bats themselves are not at fault, they live in harmony and health with viruses, and it is a variety of human activities that have altered the environment, resulting in more and more bats being infected. of animal viruses infecting humans.
Related Topics:Focus on Wuhan novel coronavirus pneumonia outbreak
In addition to revealing a lot of important information to us in this interview Wang Linfa sent out, most notably through a feature interview with him, this prominent scientist sent out a message, the same message that the article about Discovery of Inhibitors sends, the new coronavirus is not Synthesized, it is from bats in nature, and the SARS he studied in 2005 originated from bats, the same conclusion, and there is also the conclusion that the Chinese government is not responsible, with the WHO as proof. Three SARS virus lab leaks happened in 2004, this one still could be a lab leak. And because of the 2004 SARS virus leak, the research of the SARS virus came to a complete halt.
Given that we have already established that the virus must have originated in a laboratory synthesis, why does Wang Linfa still claim the new coronavirus from bats? It’s clear why his team tried to find the inhibitor. It takes a thousand lies to cover up one lie.
Let’s continue to dig into this Wang Linfa. Wang Linfa was first featured in the SARS virus study and also in the 2016 swine fever trial (see previous post). Who are the key researchers and institutions that appeared in the 2003 SARS virus study and the 2016 swine fever trial? Let’s move the earlier relevant tables here.
List of all coronaviruses discovered after 2003
Research on coronavirus in the CAS system after the 2003 SARS outbreak
Members of the Academic Committee of State Key Laboratory of Virology
The three tables reveal the secret: if the report of discovery of inhibitors of Wang Linfa and Tsinghua University is a deliberate cover-up, Wang Linfa with a high status and position to publish such arguably boring articles to deceive people who don’t understand science can only be interpreted as they started to feel guilty and distract public attention. Wang Linfa’s move to Singapore in 2012 is noteworthy: in 2012, Wang moved to Singapore and became director of Duke-NUS Medical School.
The Hong Kong team that appears in the first table with the most coronavirus discoveries consist of two teams. One is led by Thomas Kwan from the University of Hong Kong, who is in the Academic Committee’s list, another team is the same team we dug up in our swine fever outbreak article, led by Yuan Guoyong: State Key Laboratory of Emerging Infectious Diseases, Li Ka Shing Faculty of Medicine, University of Hong Kong and the Pasteur Institute, Hong Kong.
In the academic committee, Chen Yinghua of Tsinghua University was a student of the virus department of Wuhan University, and eventually became the CCP secretary of Tsinghua University’s School of Life. Zhang Linqi worked at the School of Medicine of Tsinghua University. In the memory article of Guo Deyin, he once mentioned Tian Bo’s Referral for Zhang Linqi. Tsinghua University’s role in the research of the antidote team and the true leader have surfaced. Tsinghua University and the Institute of Biophysics of the Chinese Academy of Sciences and Rao Zihe’s research are all related to the research of inhibitors. In this list, there is another person, Zhang Xianen, who also appeared in the list of researchers of Wuhan Institute of Virology. The fact that Tsinghua University, Wang Linfa, the Institute of Biophysics of the Chinese Academy of Sciences, Pasteur of Hong Kong, and the University of Hong Kong has repeatedly studied the same type of virus can only show one thing: there is a huge plan.
Let’s look at the four professors and academicians in Shanghai: Wang Hongyang, Chen Kaixian, Zhao Guoping and Yuan Zhenghong. In the following reports, they will appear in order: Note that Wang Xiaofan, a professor at Duke University, will also appear on this list. That is the American partner in the inhibitor article above, Duke University.
On October 22, 2016, the 80th Anniversary Conference of the School of Pharmacy of Fudan University was held in the Auditorium of the Administration Building, Zhangjiang Campus. It was held grandly. More than a thousand alumni from domestic and overseas gathered together with guests and teachers and students to celebrate the 80th birthday of the College of Pharmacy. Along with the commemorative meeting, there were also a concert, a frontier science summit forum, a forum on pharmacy regulatory science, a forum on pharmacy career development, and a forum on the development of pharmacy management. The forum and the hospital’s cultural evening show are among the many activities.
Guests who came to congratulate and attend the commemorative meeting included: Chinese Academy of Engineering Academian Mr. Sang Guowei, Chairman of the Shanghai Association for Science and Technology, Academician of Chinese Academy of Sciences Chen Kaixian, Chinese Academy of Engineering. Academicians Li Dapeng and Chen Fenghua, Academicians Zhao Guoping and Zhang Xuemin from the Chinese Academy of Sciences, Professor Wang Xiaofan from Duke University, and Chairman of the Board Cui Xiangmin De Cheng Capital, Chairman of the Board Li Ge of WuXi AppTec and General Manager of Aisen Bio-technology (Hangzhou) Co. Xu Xiao and other academic leaders and successful innovators and entrepreneurs; Gong Ping, Vice President of Shenyang Pharmaceutical University; Zhou Demin, Dean of School of Pharmacy, Peking University; Hao Haiping, Dean of School of Pharmacy, China Pharmaceutical University; Fang Yun, CCP Secretary of Huaxi School of Pharmacy, Sichuan University; Liu Xinyong, Dean of School of Pharmacy, Shandong University; Lou Xiao’e, CCP Secretary of School of Pharmacy, Zhejiang University; Hu Wenhao, Dean of School of Pharmacy, Sun Yat-sen University; Zhang Weidong, Executive Vice President of School of Pharmacy, Second Military Medical University; Lu Weigen, Vice President of China Pharmaceutical Industry Research Institute; and Shi Yansen, Director of Office, Shanghai Institute of Pharmaceutical Sciences, Chinese Academy of Sciences, and other representatives of sister colleges and research institutes; Chen Liang, General Manager of Agilent Technologies Greater China, Li Dongming, General Manager of Shanghai Pharmaceutical Group Pharmaceutical Sales, Yu Kunjiao, Manager of New Business Development Department of Shanghai Sino-Shanghai 3D Pharmaceutical Co, PerkinElmer’s Asia Pacific Regional Director Jie Min Yan and other business representatives; Zhang Zhiyong, Vice President of Fudan University, Xia Jinglin, Vice Dean of Shanghai Medical College of Fudan University, and Jingmin Chen, Party Secretary of the Institute of Brain Science, SMC Alumni Association President Peng Yuwen, Vice President Cheng Gang, and SMC Alumni Association Pharmacy Branch President Xiong Bingjie, and other university leaders and representatives from the university’s institutions.
“Frontier Science Summit” invites academic presentations by scientific elites, they were Zhao Guoping from Chinese Academy of Sciences, Wang Hongyang from Chinese Academy of Engineering, Ning Guang from Chinese Academy of Engineering, Shi Yang from American Academy of Humanities and Sciences (alumnus of the Fudan University School of Pharmacy Class of 1978) and Zhang Xuemin from Chinese Academy of Sciences; “Pharmaceutical Regulatory Science Forum” was presented by Xu Jiaqi, Director of the Drug Evaluation Center of the State Food and Drug Administration and Chen Yaoshui, Deputy Director of Shanghai Food and Drug Administration; Wu Peixin, Deputy Director General of the Department of Science and Education of the National Health and Family Planning Commission participated in the forum. The “Pharmacy Career Development Forum” was held in the form of a dialogue between five alumni who have achieved outstanding results in different industries, namely Li Jiebing, Investment Director of Shanghai Health Care Industry Investment Fund, Li Yaping and Xuan Lijiang, Researchers of Shanghai Institute of Pharmaceutical Sciences, Chinese Academy of Sciences, Xue Zheng, General Manager of Shanghai BAU Medical Laboratory, and Yang Jingrui, CEO of Shanghai Bikai Pharmaceutical Technology Co.
Yuan Zhenghong, is the CCP Secretary of Shanghai Medical College of Fudan University.
The collective appearance of Shanghai-based academicians and professors on the academic committee list of the State Key Laboratory of Virology at Wuhan University through this 2016 celebration and the fact that Novo Nordisk donated a library of 500,000 sample small molecule compounds to the Shanghai Institute of Pharmaceutical Sciences of the Chinese Academy of Sciences at the beginning of 2007 suggests one thing, the real big bosses can only be Jiang Mianheng and the Jiang family behind him, and the main body of the Chinese Communist Party’s state-stealing syndicate we commonly refer to as the Shanghai Gang.
Strictly speaking, the Shanghai Gang is inaccurate, because Jiang Mianheng represents not only the interests of the Jiang family, the interests of that group of people in Shanghai, but also the interests of the entire Chinese Communist Party core group, the implementation of the shipwreck plan is not just on behalf of individuals, but the organization. The organization’s ruling structure is highly centralized, this is the CCP rules, or can be understood as under the leadership of a triad boss, while the sharing of booty is necessary.
So Wuhan Institute of Virology, the core of inhibitor development, Australia’s Wang Fu Lin, National University of Singapore, Li Ka-shing Medicine Center for Emerging Infectious Diseases of Hong Kong University, Hong Kong Pasteur, as well as Shanghai Pasteur all point to this manipulator behind the scene, now, more fields can be added to the previous table:
Biological Weapons Super Squad
If this form is true, it will be a truly astounding secret, then is this the core layout and core secret of P4 Labs?
Please listen to the rest of the story next time.
Edited by 【Himalaya Hawk Squad】